Coding
prhA

Part:BBa_K367000:Design

Designed by: Fernando Govantes   Group: iGEM10_UPO-Sevilla   (2010-06-29)

prhA coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 658
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 724
    Illegal AgeI site found at 928
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Stop codon changed to TAATAA. Codon usage adapted to Escherichia coli, using Mr. Gene's tools. Purchased from Mr. Gene. Sequenced in origin.

Source

Ralstonia solanacearum GMI1000 plasmid pGMI1000MP, complete sequence NCBI Reference Sequence: NC_003296.1

References